Journal: Journal of Sport and Health Science

Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice

doi: 10.1016/j.jshs.2025.101095

Figure Lengend Snippet: Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.

Article Snippet: Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

Techniques: Expressing, Western Blot, Isolation, Marker, Control, Membrane

Journal: Frontiers in Physiology

Article Title: Integrated proteomics and metabolomics reveal mechanisms of blood pressure reduction in spontaneously hypertensive rats under hypoxic conditions

doi: 10.3389/fphys.2026.1859072

Figure Lengend Snippet: Changes in the protein expression levels of cytochrome c oxidase subunit 5A (Cox5a), NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1), ubiquinol-cytochrome c reductase core protein 2 (Uqcrc2), carnitine palmitoyltransferase (Cpt2), acyl-CoA synthetase long chain family member 1 (AcsI1), and kininogen 1 (Kng1) in the abdominal aorta of spontaneously hypertensive rats (SHR) after exposure to high-altitude hypoxic conditions. (A) Western blot images. Corresponding densitometric quantification. Band intensity was normalized to β−actin and quantified using Gel−Pro Analyzer 4 software. (B) Cox5a. (C) Ndufs1. (D) Uqcrc2. (E) Cpt2. (F) Kng1. (G) AcsI1. Data are shown as the mean ± SD (n = 3). SHR-C and SHR-H refer to the SHR control group and SHR high-altitude hypoxia group, respectively. * P < 0.05 vs. SHR-C ( t -test).

Article Snippet: Anti-β-actin, cytochrome c oxidase subunit 5A (Cox5a), kininogen 1 (Kng1), and NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1) antibodies were obtained from Abclonal (Wuhan, China), whereas anti-acyl-coenzyme (CoA) synthetase long-chain family member 1 (AcsI1) and carnitine palmitoyltransferase 2 (Cpt2) antibodies were obtained from Huabio (Hangzhou, China).

Techniques: Expressing, Western Blot, Software, Control